1 Search Results for "Hruz, Tomas"


Document
Improved De Novo Peptide Sequencing using LC Retention Time Information

Authors: Yves Frank, Tomas Hruz, Thomas Tschager, and Valentin Venzin

Published in: LIPIcs, Volume 88, 17th International Workshop on Algorithms in Bioinformatics (WABI 2017)


Abstract
Liquid chromatography combined with tandem mass spectrometry (LC-MS/MS) is an important tool in proteomics for identifying the peptides in a sample. Liquid chromatography temporally separates the peptides and tandem mass spectrometry analyzes the peptides, that elute one after another, by measuring their mass-to-charge ratios and the mass-to-charge ratios of their prefix and suffix fragments. De novo peptide sequencing is the problem of reconstructing the amino acid sequences of the analyzed peptide from this measurement data. While previous approaches solely consider the mass spectrum of the fragments for reconstructing a sequence, we propose to also exploit the information obtained from liquid chromatography. We study the problem of computing a sequence that is not only in accordance with the experimental mass spectrum, but also with the retention time of the separation by liquid chromatography. We consider three models for predicting the retention time of a peptide and develop algorithms for de novo sequencing for each model. An evaluation on experimental data from synthesized peptides for two of these models shows an improved performance compared to not using the chromatographic information.

Cite as

Yves Frank, Tomas Hruz, Thomas Tschager, and Valentin Venzin. Improved De Novo Peptide Sequencing using LC Retention Time Information. In 17th International Workshop on Algorithms in Bioinformatics (WABI 2017). Leibniz International Proceedings in Informatics (LIPIcs), Volume 88, pp. 26:1-26:17, Schloss Dagstuhl – Leibniz-Zentrum für Informatik (2017)


Copy BibTex To Clipboard

@InProceedings{frank_et_al:LIPIcs.WABI.2017.26,
  author =	{Frank, Yves and Hruz, Tomas and Tschager, Thomas and Venzin, Valentin},
  title =	{{Improved De Novo Peptide Sequencing using LC Retention Time Information}},
  booktitle =	{17th International Workshop on Algorithms in Bioinformatics (WABI 2017)},
  pages =	{26:1--26:17},
  series =	{Leibniz International Proceedings in Informatics (LIPIcs)},
  ISBN =	{978-3-95977-050-7},
  ISSN =	{1868-8969},
  year =	{2017},
  volume =	{88},
  editor =	{Schwartz, Russell and Reinert, Knut},
  publisher =	{Schloss Dagstuhl -- Leibniz-Zentrum f{\"u}r Informatik},
  address =	{Dagstuhl, Germany},
  URL =		{https://drops-dev.dagstuhl.de/entities/document/10.4230/LIPIcs.WABI.2017.26},
  URN =		{urn:nbn:de:0030-drops-76383},
  doi =		{10.4230/LIPIcs.WABI.2017.26},
  annote =	{Keywords: Computational proteomics, Peptide identification, Mass spectrometry, De novo peptide sequencing, Retention time prediction}
}
  • Refine by Author
  • 1 Frank, Yves
  • 1 Hruz, Tomas
  • 1 Tschager, Thomas
  • 1 Venzin, Valentin

  • Refine by Classification

  • Refine by Keyword
  • 1 Computational proteomics
  • 1 De novo peptide sequencing
  • 1 Mass spectrometry
  • 1 Peptide identification
  • 1 Retention time prediction

  • Refine by Type
  • 1 document

  • Refine by Publication Year
  • 1 2017

Questions / Remarks / Feedback
X

Feedback for Dagstuhl Publishing


Thanks for your feedback!

Feedback submitted

Could not send message

Please try again later or send an E-mail