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Documents authored by Tholey, Andreas

Document
DOI: 10.4230/OASIcs.GCB.2013.56
Efficient Interpretation of Tandem Mass Tags in Top-Down Proteomics

Authors: Anna Katharina Hildebrandt, Ernst Althaus, Hans-Peter Lenhof, Chien-Wen Hung, Andreas Tholey, and Andreas Hildebrandt

Published in: OASIcs, Volume 34, German Conference on Bioinformatics 2013

Abstract
Mass spectrometry is the major analytical tool for the identification and quantification of proteins in biological samples. In so-called top-down proteomics, separation and mass spectrometric analysis is performed at the level of intact proteins, without preparatory digestion steps. It has been shown that the tandem mass tag (TMT) labeling technology, which is often used for quantification based on digested proteins (bottom-up studies), can be applied in top-down proteomics as well. This, however, leads to a complex interpretation problem, where we need to annotate measured peaks with their respective generating protein, the number of charges, and the a priori unknown number of TMT-groups attached to this protein. In this work, we give an algorithm for the efficient enumeration of all valid annotations that fulfill available experimental constraints. Applying the algorithm to real-world data, we show that the annotation problem can indeed be efficiently solved. However, our experiments also demonstrate that reliable annotation in complex mixtures requires at least partial sequence information and high mass accuracy and resolution to go beyond the proof-of-concept stage.
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Anna Katharina Hildebrandt, Ernst Althaus, Hans-Peter Lenhof, Chien-Wen Hung, Andreas Tholey, and Andreas Hildebrandt. Efficient Interpretation of Tandem Mass Tags in Top-Down Proteomics. In German Conference on Bioinformatics 2013. Open Access Series in Informatics (OASIcs), Volume 34, pp. 56-67, Schloss Dagstuhl – Leibniz-Zentrum für Informatik (2013)

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@InProceedings{hildebrandt_et_al:OASIcs.GCB.2013.56,
  author =	{Hildebrandt, Anna Katharina and Althaus, Ernst and Lenhof, Hans-Peter and Hung, Chien-Wen and Tholey, Andreas and Hildebrandt, Andreas},
  title =	{{Efficient Interpretation of Tandem Mass Tags in Top-Down Proteomics}},
  booktitle =	{German Conference on Bioinformatics 2013},
  pages =	{56--67},
  series =	{Open Access Series in Informatics (OASIcs)},
  ISBN =	{978-3-939897-59-0},
  ISSN =	{2190-6807},
  year =	{2013},
  volume =	{34},
  editor =	{Bei{\ss}barth, Tim and Kollmar, Martin and Leha, Andreas and Morgenstern, Burkhard and Schultz, Anne-Kathrin and Waack, Stephan and Wingender, Edgar},
  publisher =	{Schloss Dagstuhl -- Leibniz-Zentrum f{\"u}r Informatik},
  address =	{Dagstuhl, Germany},
  URL =		{https://drops.dagstuhl.de/entities/document/10.4230/OASIcs.GCB.2013.56},
  URN =		{urn:nbn:de:0030-drops-42304},
  doi =		{10.4230/OASIcs.GCB.2013.56},
  annote =	{Keywords: Mass spectrometry, TMT labeling, Top-down Proteomics}
}
Document
DOI: 10.4230/DagSemProc.05471.10
MALDI Mass Spectrometry for Quantitative Proteomics – Approaches, Scopes and Limitations

Authors: Andreas Tholey

Published in: Dagstuhl Seminar Proceedings, Volume 5471, Computational Proteomics (2006)

Abstract
The determination of absolute protein amounts and the quantification of differentially expressed proteins belong to the most important goals in proteomics. Despite being one of the key technologies for the identification of proteins, the application of matrix assisted laser desorption/ionization (MALDI) mass spectrometry (MS) for quantitative analyses is hampered by several inherent factors. The goal of the present paper is to outline these difficulties but also to present some selected approaches which enable MALDI MS to be used for the quantification of biomolecules. In particular, methods for the improvement of the homogeneity of MALDI samples and the use of internal standards for the relative quantification are discussed. Strategies for in-vivo and in-vitro labelling of peptides and proteins with stable isotopes are presented. The need for guidelines for the presentation and evaluation of data as well as for bioinformatical approaches for the interpretation of quantitative data will be addressed.
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Andreas Tholey. MALDI Mass Spectrometry for Quantitative Proteomics – Approaches, Scopes and Limitations. In Computational Proteomics. Dagstuhl Seminar Proceedings, Volume 5471, pp. 1-11, Schloss Dagstuhl – Leibniz-Zentrum für Informatik (2006)

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@InProceedings{tholey:DagSemProc.05471.10,
  author =	{Tholey, Andreas},
  title =	{{MALDI Mass Spectrometry for Quantitative Proteomics – Approaches, Scopes and Limitations}},
  booktitle =	{Computational Proteomics},
  pages =	{1--11},
  series =	{Dagstuhl Seminar Proceedings (DagSemProc)},
  ISSN =	{1862-4405},
  year =	{2006},
  volume =	{5471},
  editor =	{Christian G. Huber and Oliver Kohlbacher and Knut Reinert},
  publisher =	{Schloss Dagstuhl -- Leibniz-Zentrum f{\"u}r Informatik},
  address =	{Dagstuhl, Germany},
  URL =		{https://drops.dagstuhl.de/entities/document/10.4230/DagSemProc.05471.10},
  URN =		{urn:nbn:de:0030-drops-5367},
  doi =		{10.4230/DagSemProc.05471.10},
  annote =	{Keywords: MALDI, mass spectrometry, quantification, ionic liquid matrices}
}
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